Volume 5, Issue 4, July 2017, Page: 102-111
Effects of Gibberellic Acid Responsive Dwarfing Gene Rht9 on Plant Height and Agronomic Traits in Common Wheat
Tay Zar Linn, State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, P. R. China
Daoura Goudia Bachir, State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, P. R. China
Liang Chen, State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, P. R. China
Yin-Gang Hu, State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, P. R. China; Institute of Water Saving Agriculture in Arid Regions of China, Yangling, P. R. China
Received: May 11, 2017;       Accepted: May 20, 2017;       Published: Jul. 7, 2017
DOI: 10.11648/j.ajaf.20170504.14      View  2623      Downloads  68
Abstract
To explore the potential use of GA-responsive dwarfing gene Rht9 in common wheat breeding program, its effects on plant height, seedling vigor, photosynthesis and yield traits were investigated and compared in field experiments using hexaploid Rht9 dwarf lines derived from two crosses of Chinese winter wheat cultivars Xifeng 20 and Jinmai 47 with the Rht9 tetraploid donor Granaoto. Xifeng20-Rht9 dwarf lines reduced plant height on average by 25.38%, while on average by 9.39% in Jinmai47-Rht9/Rht8 dwarf lines. Compared with taller parents, coleoptile length was reduced by 19.80% in Xifeng20-Rht9 dwarf lines, while it was increased by 14.22% in Jinmai 47-Rht9/Rht8 dwarf lines. There were no adverse effects of Rht9 on root characters and flag leaf characters, though slightly increased relative leaf chlorophyll content (SPAD) observed. Grain numbers per spike was increased on average by 19.63%, and biomass per plant was slightly decreased on average by 3.37% in Xifeng 20-Rht9 dwarf lines, while, grain number per spike was decreased on average by 11.49%, and biomass per plant was increased on average by 8.57% in Jinmai47-Rht9/Rht8 dwarf lines. Compared with taller parents, Rht9 increased fertile tillers on average by 11.25% and 11.19%, grain yield on average by 10.11% and 14.10%, harvest index on average by 12.67% and 6.85%, while decreased spike length on average by 4.80% and 16.23%, slightly decreased 1000 kernels weight by 4.43% and 4.61%, in the Rht9 dwarf lines of Xifeng 20 and Jinmai 47, respectively. The results of current study could be useful for proper use of dwarfing gene Rht9 to improve lodging resistance, grain yield potential in wheat breeding programs for water limited area.
Keywords
Dwarfing Genes, Rht8, Rht9, Plant Height, Seedling Vigor, Yield, Wheat
To cite this article
Tay Zar Linn, Daoura Goudia Bachir, Liang Chen, Yin-Gang Hu, Effects of Gibberellic Acid Responsive Dwarfing Gene Rht9 on Plant Height and Agronomic Traits in Common Wheat, American Journal of Agriculture and Forestry. Vol. 5, No. 4, 2017, pp. 102-111. doi: 10.11648/j.ajaf.20170504.14
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
A. Goyal, R. Prasad, Some important fungal diseases and their impact on wheat production. in: A. Arya, A. Perello (Eds). Management of Fungal Plant Pathogens, CABI, ISBN 9781845936037, 2010, 362.
[2]
P. Hedden, The genes of the Green Revolution, Trends in Genetics. 19, 2003, 5–9.
[3]
M. D. Gale, S. Youssefian, Dwarfing genes in wheat, in: GE Russed (Eds), Progress in Plant Breeding. Vol 1, Butterworths, London, 1985, 1–35.
[4]
K. B. Borojevic, The transfer and history of 'reduced height genes' (Rht) in wheat from Japan to Europe, Journal of Heredity. 96, 2005, 455-459.
[5]
J. B. Reid, Gibberellin mutants. in: Blonstein AD, King PJ (EDs). Plant Gene Research, a Genetic Approach to Plant Biochemistry, New York, Springer-Verlag, 1986, 1–34.
[6]
A. J. Worland, V. Korzum, M. S. Roder, M. W. Ganal, C. N. Law, Genetic analysis of the dwarfing gene (Rht8) in wheat. Part II, the distribution and adaptive significance of allelic variants at Rht8 locus of wheat as revealed by microsatellite screening, Theor Appl Genet. 96, 1998, 1110–1120.
[7]
G. J. Rebetzke, R. Appels, A. Morrison, R. A. Richards, G. Mc Donald, M. H. Ellis, W. Spielmeyer, D. G. Bonnett, Quantitative trait loci on chromosome 4B for coleoptile length and early vigour in wheat (Triticum aestivum L.), Aust J Agric Res. 52, 2001, 1221–1234.
[8]
J. Hoogendoorn, J. M. Rickson, M. D. Gale, Differences in leaf and stem anatomy related to plant height of tall and dwarf wheat, J Plant Phys. 136, 1990, 72–77.
[9]
J. D. Butler, P. F. Byrne, V. Mohammadi, P. L. Chapman, S. D. Haley, Agronomic performance of Rht alleles in a spring wheat population across a range of moisture levels, Crop Sci. 45, 2005, 939–947.
[10]
K. L. Mathews, S. C. Chapman, R. Trethowan, R. P. Singh, J. Crossa, W. Pfeiffer, M. van Ginkel, I. De Lacy, Global adaptation of spring bread and durum wheat lines nearisogenic for major reduced height genes, Crop Sci. 46, 2006, 603–613.
[11]
G. J. Rebetzke, R. A. Richards, N. A. Fettell, M. Long, A. G. Condon, R. I. Forrester, T. L. Botwright, Genotypic increases in coleoptile length improves stand establishment, vigour and grain yield of deep-sown wheat, Field Crops Research. 100, 2007, 10-23.
[12]
R. E. Allan, Agronomic comparisons between Rht1 and Rht2 semi dwarf genes in winter wheat, Crop Sci. 29, 1989, 1103-1108.
[13]
G. J. Rebetzke, R. A. Richards, Gibberellic acid-sensitive dwarfing genes reduce plant height to increase kernel number and grain yield of wheat, Aust J Agric Res. 512, 2000, 235–245.
[14]
W. F. Schillinger, E. Donaldson, R. E. Allan, S. S. Jones, Winter wheat seedling emergence from deep sowing depths, Agron J. 90, 1998, 582–586.
[15]
G. J. Rebetzke, R. A. Richards, V. M. Fischer, B. J. Mickelson, Breeding long coleoptile, reduced height wheat, Euphytica. 106, 1999, 159–168.
[16]
G. J. Rebetzke, R. A. Richards, X. R. R. Sirault, A. D. Morrison, Genetic analysis of coleoptile length and diameter of wheat, Aust J Agric Res. 55, 2004, 733-743.
[17]
M. H. Ellis, G. J. Rebetzke, A. G. Condon, W. Spielmeyer, R. A. Richards, The effect of different height reducing genes on early growth characteristics of wheat, Funct Plant Biol. 31, 2004, 583–589.
[18]
M. H. Ellis, G. J. Rebetzke, F. Azanza, R. A. Richards, W. Spielmeyer, R. A. Richards, Molecular mapping of GR dwarfing genes in bread wheat, Theor Appl Genet. 111, 2005, 423-430.
[19]
C. F. Konzak, Mutations and mutation breeding, In: Heyne, E. C. (Ed.), Wheat and Wheat Improvement, American Society of Agronomy, Madison, WI, 1987, 428–443.
[20]
N. P. Loskutova, The influence of Rht 1–5, Rht 8–9 and Rht 13 genes on morphological characters and yield productivity of wheat. in: Slinkard, A. E. (Ed.), Proceedings of the 9th International Wheat Genetics Symposium, University Extension Press, University Saskatchewan, Saskatoon, 1998, 283–284.
[21]
Y. Wang, C. Liang, D. Yingying, Zy. Yang, A. G. Condon, Y. G. Hu, Genetic effect of dwarfing gene Rht13 compared with Rht-D1b on plant height and some agronomic traits in common wheat (Triticum aestivum L.), Field Crops Research. 162, 2014, 39-47.
[22]
Zy. Yang, J. Zheng, C. Liu, Y. Wang,, A. G. Condon, Y. Chen, Y. G. Hu, Effects of the GA-responsive dwarfing gene Rht18 from tetraploid wheat on agronomic traits of common wheat, Field Crops Research. 183, 2015, 92-101.
[23]
M. H. Ellis, W. Spielmeyer, K. R. Gale, G. J. Rebetzke, R. A. Richards, Perfect markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat, Theor Appl Genet. 105 (2002) 1038–1042.
[24]
M. S. Clark, Plant Molecular Biology: A laboratory Manual, Springer-Verlag, Berlin Heidelberg, New York, 1997, 305-328.
[25]
V. Korzun, M. S. Roder, M. W. Ganal, A. J. Worland, C. N. Law, Genetic analysis of the dwarfing gene (Rht8) in wheat, Part I. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticum aestivum L.), Theor Appl Genet, 96, 1998, 1104–1109.
[26]
B. J. Bassam, G. Caetano-Anolles, P. M. Gresshoff, Fast and sensitive silver staining of DNA in polyacrylamide gels, Anal Biochem. 196, 1991, 80-83.
[27]
J. C. Zadoks, T. T. F. C. Chang, A decimal code for the growth stages of cereals, Weed Res. 14, 1974, 415–421.
[28]
G. Lobet, L. Pages, X. Draye, A novel image-analysis toolbox enabling quantitative analysis of root system architecture, Plant Physiol. 157, 2011, 29–39.
[29]
H. Hu, Y. L. Bai, L. P. Yang, Y. L. Lu, L. Wang, H. Wang, Z. Y. Wang, Diagnosis of nitrogen nutrition in winter wheat (Triticum aestivum) via SPAD-502 and GreenSeeker, Chin. J. Eco-Agric. 18, 2010, 748–752.
[30]
T. L. Botwright, G. J. Rebetzke, A. G. Condon, R. A. Richards, The effect of rht genotype and temperature on coleoptile growth and dry matter partitioning in young wheat seedlings, Aust J Plant Physiol. 15, 2001, 417–423.
[31]
G. J. Rebetzke, M. H. Ellis, D. G. Bonnett, B. Mickelson, A. G. Condon, R. A. Richards, Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.), Field Crops Research. 126, 2012, 87-96.
[32]
R. B. Austin, Yield of wheat in the United Kingdom: Recent advances and prospects, Crop Science. 39 (6), 1999, 1604-1610.
[33]
J. E. Flintham, A. Börner, A. J. Worland, M. D. Gale, Optimizing wheat grain yield: Effects of Rht (gibberellin-insensitive) dwarfing genes, Journal of Agricultural Science. 128 (1), 1997, 11-25.
[34]
L. Chen, A. L. Phillips, A. G. Condon, M. A. J. Parry, Y. G. Hu, GA-Responsive Dwarfing Gene Rht12 Affects the Developmental and Agronomic Traits in Common Bread Wheat, PLOS ONE. 8 (4), 2013, e62285.
[35]
F. G. H. Lupton, R. H. Oliver, F. B. Ellis, B. T. Barnes, K. R. Howse, P. J. Welbank, P. J. Taylor, Root and shoot growth of semi-dwarf and taller winter wheats, Ann Appl Biol. 77, 1974, 129–144.
[36]
T. Wojciechowski, M. J. Gooding, L. Ramsay, P. J. Gregory, The effects of dwarfing genes on seedling root growth of wheat, J Exp Bot. 60, 2009, 2565–2573.
[37]
K. H. M. Siddique, R. K. Belford, D. Tennant, Root: shoot ratios of old and modern, tall and semi dwarf wheats in a Mediterranean environment, Plant Soil. 121, 1990, 89–98.
[38]
D. J. Miralles, G. A. Slafer, V. Lynch, Rooting patterns in near-isogenic lines of spring wheat for dwarfism, Plant Soil. 197, 1997, 79–86.
[39]
R. B. Austin, J. A. Edrich, L. T. Evans, M. A. Ford, R. D. Blackwell, The fate of the dry weight, carbohydrates and C14 lost from the leaves and stems of wheat during grain filling, Ann Bot. 41, 1977, 1309–1321.
[40]
M. P. Reynolds, M. Balota, M. I. B. Delgado, I. Amani, R. A. Fischer, Physiological and morphological traits associated with spring wheat yield under hot, irrigated conditions, Aust J Plant Physiol. 21, 1994, 717–730.
[41]
B. G. Daoura, L. Chen, Y. Du, Y. G. Hu, Genetic effects of dwarfing gene Rht-5 on agronomic traits in common wheat (Triticum aestivum L.) and QTL analysis on its linked traits, Field Crops Research, 2014, 22–29.
[42]
Y. Wang, Y. Du, Zy. Yang, L. Cheng, A. G. Condon, Y. G. Hu, Comparing the effects of GA-responsive dwarfing genes Rht13 and Rht8 on plant height and some agronomic traits in common wheat, Field Crops Research. 179, 2015, 35-43.
[43]
R. A. Fischer, Y. M. Stockman, Increased kernel number in Norin 10-derived dwarf wheat: evaluation of the cause, Aust J Plant Physiol. 13, 1986, 767–784.
[44]
M. Nizam Uddin and D. R. Marshall, Effects of dwarfing genes on yield and yield components under irrigated and rainfed condi tions in wheat (Triticum aestivum L.), Euphytica. 42, 1989, 127–134.
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