Volume 6, Issue 5, September 2018, Page: 132-140
Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants
Yuling Yin, Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China
Shaochun Luo, Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China
Yunpeng Li, College of Agricultural Resources and Environment, Heilongjiang University, Harbin, China
Jinsong Zhou, Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China
Yongping Tang, Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China
Yuan Liu, Liaoning Saline or Alkaline Land Utilization and Research Institute, Liaoning Academy of Agricultural Sciences, Panjin, China
Received: May 7, 2018;       Accepted: Aug. 7, 2018;       Published: Sep. 15, 2018
DOI: 10.11648/j.ajaf.20180605.13      View  363      Downloads  25
Abstract
This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. Verticillium dahliae, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of Verticillium wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of Verticillium dahliae in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of Verticillium dahliae growth to study the prevention and curative effects on Verticillium wilt in pot trials.
Keywords
Grafting Eggplants, Verticillium Wilt, Verticillium dahliae, Continuous Cropping, Soil Microorganism
To cite this article
Yuling Yin, Shaochun Luo, Yunpeng Li, Jinsong Zhou, Yongping Tang, Yuan Liu, Grafting shaping the Microbial Community Structure to Suppress Verticillium dahliae in the Rhizosphere of Eggplants, American Journal of Agriculture and Forestry. Vol. 6, No. 5, 2018, pp. 132-140. doi: 10.11648/j.ajaf.20180605.13
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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