Volume 8, Issue 5, September 2020, Page: 208-213
Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract
Thangamani Dhandapani, Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India
KR Sasidharan, Forest Ecology and Climate Change Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India
Girija Shanmugam, Department of Biotechnology, Bharathiar University, Coimbatore, India
Vimaladevi Sadanandam, Germplasm Conservation Division, ICAR-NBPGR, New Delhi, India
Ragunathan Vasudevan, Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India
Suryanarayanan Lalitha, Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India
Ravichandran Anandalakshmi, Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India
Received: Sep. 19, 2020;       Accepted: Sep. 28, 2020;       Published: Oct. 12, 2020
DOI: 10.11648/j.ajaf.20200805.14      View  14      Downloads  19
Abstract
Dalbergia latifolia commonly known as the Indian rosewood, commonly found in association with Teak trees population in and around Western Ghat areas of Tamil Nadu and Kerala in India. From the heartwood of roots and trunk many phytochemical contents were investigated. A wide spectrum of flavanoids namely, flavones, isoflavones, neoflavones and chalcones have been obtained. Specifically, one of the flavonoids compound chalcone compound-Isoliquiritigenin (ILTG, 2',4',4'-trihydroxychalcone) shows various pharmacological properties, including cytotoxic activities. This study revealed the cytotoxic effect of Isoliquiritigenin, efficiently controls the invasive capacity of breast cancer cells MCF10A human mammary epithelial cell line through uplifting G1-phase cell cycle, even arrest at low concentration, also accelerate the extracellular signal regulated kinase signaling pathway to topup the proteins combined with apoptosis and arrest cancer. The cytotoxicity invitro of isoliquitrigenin against MCF10A ATCC cells was investigated by MTT assay. Apoptosis was evaluated by microscopy and flow cytometry. Isoliquiritigenin exhibited the most potent anticancer activity against MCF 10A ATCC with an IC 50 of 10.00 µg/ml. The methanolic extract expressed cytotoxic effect with an IC50 value of 20 µg/ml. The compounds were also tested for their toxicity on normal human cell lines-LO2 and were found to be nontoxic. Administering the wood extract approaches may help reduce side effects in patients under conventional chemotherapy. These potential molecule may exerts their co-active effects through various pathways and helpful for the development of novel drugs; also conservation of these important germplasm is mandatory.
Keywords
Dalbergia latifolia, Isoliquiritigenin, Breast Cancer Cell Line, Cytotoxicity, Conservation
To cite this article
Thangamani Dhandapani, KR Sasidharan, Girija Shanmugam, Vimaladevi Sadanandam, Ragunathan Vasudevan, Suryanarayanan Lalitha, Ravichandran Anandalakshmi, Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract, American Journal of Agriculture and Forestry. Vol. 8, No. 5, 2020, pp. 208-213. doi: 10.11648/j.ajaf.20200805.14
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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