To obtain monosaccharide components information of Dendrobium moniliforme polysaccharides, a HPLC-ESI-MS method was proposed. The crude polysaccharides were extracted and purified from D. moniliforme as samples (DMP). Then, the polysaccharides were hydrolyzed with trifluoroacetic acid (TFA) and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). PMP-labeled mixture of monosaccharides were separated by a reverse-phase high performance liquid chromatography (HPLC) and detected by on-line electrospray ionization mass spectrometry (ESI-MS). With this method, the six monosaccharide derivatives have been well separated and the identification of the monosaccharides composition was carried out. The result showed that D. moniliforme polysaccharide consisted of D-mannose, D-ribose, D-glucose, D-galactose and L-arabinose, in a molar ratio of 51.83: 4.01: 38.90: 4.20: 1.65, respectively. Moreover, hydrolysis studies revealed that the monosaccharide concentrations reached the peak value at different time and it suggested that the release of DMP monosaccharides was not hydrolyzed at the same rate. These results indicated that the DMP may be a mixture of multiple polysaccharides. Finally, we used a high performance gel permeation chromatography (HPGPC) method proved that crude polysaccharides were composed by three kinds of polysaccharides (DMP-1, DMP-2 and DMP-3) with molecular weight of 153.1 kDa, 69.28 kDa and 50.14 kDa.
| Published in | American Journal of Agriculture and Forestry (Volume 4, Issue 6) |
| DOI | 10.11648/j.ajaf.20160406.13 |
| Page(s) | 156-162 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Dendrobium moniliforme Polysaccharide, Monosaccharide Components, High Performance Gel Permeation Chromatography (HPGPC), High-Performance Liquid Chromatography (HPLC), Electronspray Ionization-Mass Spectroscopy (ESI-MS)
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APA Style
Xingjun Xi, Shangzhen Xiao, Fei Tang, Qiao Chu, Tao Lan, et al. (2016). Monosaccharide Analysis of Dendrobium moniliforme Polysaccharides by High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry. American Journal of Agriculture and Forestry, 4(6), 156-162. https://doi.org/10.11648/j.ajaf.20160406.13
ACS Style
Xingjun Xi; Shangzhen Xiao; Fei Tang; Qiao Chu; Tao Lan, et al. Monosaccharide Analysis of Dendrobium moniliforme Polysaccharides by High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry. Am. J. Agric. For. 2016, 4(6), 156-162. doi: 10.11648/j.ajaf.20160406.13
AMA Style
Xingjun Xi, Shangzhen Xiao, Fei Tang, Qiao Chu, Tao Lan, et al. Monosaccharide Analysis of Dendrobium moniliforme Polysaccharides by High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry. Am J Agric For. 2016;4(6):156-162. doi: 10.11648/j.ajaf.20160406.13
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Download@article{10.11648/j.ajaf.20160406.13,
author = {Xingjun Xi and Shangzhen Xiao and Fei Tang and Qiao Chu and Tao Lan and Genlai Dong},
title = {Monosaccharide Analysis of Dendrobium moniliforme Polysaccharides by High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry},
journal = {American Journal of Agriculture and Forestry},
volume = {4},
number = {6},
pages = {156-162},
doi = {10.11648/j.ajaf.20160406.13},
url = {https://doi.org/10.11648/j.ajaf.20160406.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20160406.13},
abstract = {To obtain monosaccharide components information of Dendrobium moniliforme polysaccharides, a HPLC-ESI-MS method was proposed. The crude polysaccharides were extracted and purified from D. moniliforme as samples (DMP). Then, the polysaccharides were hydrolyzed with trifluoroacetic acid (TFA) and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). PMP-labeled mixture of monosaccharides were separated by a reverse-phase high performance liquid chromatography (HPLC) and detected by on-line electrospray ionization mass spectrometry (ESI-MS). With this method, the six monosaccharide derivatives have been well separated and the identification of the monosaccharides composition was carried out. The result showed that D. moniliforme polysaccharide consisted of D-mannose, D-ribose, D-glucose, D-galactose and L-arabinose, in a molar ratio of 51.83: 4.01: 38.90: 4.20: 1.65, respectively. Moreover, hydrolysis studies revealed that the monosaccharide concentrations reached the peak value at different time and it suggested that the release of DMP monosaccharides was not hydrolyzed at the same rate. These results indicated that the DMP may be a mixture of multiple polysaccharides. Finally, we used a high performance gel permeation chromatography (HPGPC) method proved that crude polysaccharides were composed by three kinds of polysaccharides (DMP-1, DMP-2 and DMP-3) with molecular weight of 153.1 kDa, 69.28 kDa and 50.14 kDa.},
year = {2016}
}
TY - JOUR T1 - Monosaccharide Analysis of Dendrobium moniliforme Polysaccharides by High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry AU - Xingjun Xi AU - Shangzhen Xiao AU - Fei Tang AU - Qiao Chu AU - Tao Lan AU - Genlai Dong Y1 - 2016/12/07 PY - 2016 N1 - https://doi.org/10.11648/j.ajaf.20160406.13 DO - 10.11648/j.ajaf.20160406.13 T2 - American Journal of Agriculture and Forestry JF - American Journal of Agriculture and Forestry JO - American Journal of Agriculture and Forestry SP - 156 EP - 162 PB - Science Publishing Group SN - 2330-8591 UR - https://doi.org/10.11648/j.ajaf.20160406.13 AB - To obtain monosaccharide components information of Dendrobium moniliforme polysaccharides, a HPLC-ESI-MS method was proposed. The crude polysaccharides were extracted and purified from D. moniliforme as samples (DMP). Then, the polysaccharides were hydrolyzed with trifluoroacetic acid (TFA) and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). PMP-labeled mixture of monosaccharides were separated by a reverse-phase high performance liquid chromatography (HPLC) and detected by on-line electrospray ionization mass spectrometry (ESI-MS). With this method, the six monosaccharide derivatives have been well separated and the identification of the monosaccharides composition was carried out. The result showed that D. moniliforme polysaccharide consisted of D-mannose, D-ribose, D-glucose, D-galactose and L-arabinose, in a molar ratio of 51.83: 4.01: 38.90: 4.20: 1.65, respectively. Moreover, hydrolysis studies revealed that the monosaccharide concentrations reached the peak value at different time and it suggested that the release of DMP monosaccharides was not hydrolyzed at the same rate. These results indicated that the DMP may be a mixture of multiple polysaccharides. Finally, we used a high performance gel permeation chromatography (HPGPC) method proved that crude polysaccharides were composed by three kinds of polysaccharides (DMP-1, DMP-2 and DMP-3) with molecular weight of 153.1 kDa, 69.28 kDa and 50.14 kDa. VL - 4 IS - 6 ER -
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