Using Fluorescence in Situ Hybridization with 45S rDNA probes, we analyzed two wild rice species, Oryza alta and Oryza latifolia, both having CCDD genomes. The results showed that the hybridization signals of 45S rDNA in O.latifolia chromosome preparations were distributed on different chromosomes with the number of 10~16. The same signals in O.alta chromosome preparations were 6 and distributed on three pairs of homologous chromosomes: with two pairs in short chromosome arms and the other one pair in long chromosome arms. The number and positions of 45S rDNA signals are stable in O.alta genome, but there are some dynamic changes of signals in O.latifolia genome. Our observations indicated that there are distinct differences between the two CCDD genomes. Comparative analysis of the karyotype of O.alta and O.latifolia chromosomes based on the Fluorescence in Situ Hybridization pictures showed vast differences. We propose that the genome of O.alta may be differentiated earlier in evolution and tend to be stable, while O.latifolia may still be in its evolutionary process. Based on the imbalance of evolution and differences of genomic structure, we propose that O.alta and O.latifolia can be divided into two wild rice species, which may be better conforming with their evolution characteristics. The mechanism and characteristics of 45s rDNA’s distribution in the chromosomes are discussed.
| Published in | American Journal of Agriculture and Forestry (Volume 6, Issue 6) |
| DOI | 10.11648/j.ajaf.20180606.12 |
| Page(s) | 169-175 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
45S rDNA, Fluorescence in Situ Hybridization (FISH), Oryza alta, Oryza latifolia
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APA Style
Zhou Hui, Lan Weizhen, Qin Rui, Liu Hong, Li Gang. (2018). FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes. American Journal of Agriculture and Forestry, 6(6), 169-175. https://doi.org/10.11648/j.ajaf.20180606.12
ACS Style
Zhou Hui; Lan Weizhen; Qin Rui; Liu Hong; Li Gang. FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes. Am. J. Agric. For. 2018, 6(6), 169-175. doi: 10.11648/j.ajaf.20180606.12
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Download@article{10.11648/j.ajaf.20180606.12,
author = {Zhou Hui and Lan Weizhen and Qin Rui and Liu Hong and Li Gang},
title = {FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes},
journal = {American Journal of Agriculture and Forestry},
volume = {6},
number = {6},
pages = {169-175},
doi = {10.11648/j.ajaf.20180606.12},
url = {https://doi.org/10.11648/j.ajaf.20180606.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20180606.12},
abstract = {Using Fluorescence in Situ Hybridization with 45S rDNA probes, we analyzed two wild rice species, Oryza alta and Oryza latifolia, both having CCDD genomes. The results showed that the hybridization signals of 45S rDNA in O.latifolia chromosome preparations were distributed on different chromosomes with the number of 10~16. The same signals in O.alta chromosome preparations were 6 and distributed on three pairs of homologous chromosomes: with two pairs in short chromosome arms and the other one pair in long chromosome arms. The number and positions of 45S rDNA signals are stable in O.alta genome, but there are some dynamic changes of signals in O.latifolia genome. Our observations indicated that there are distinct differences between the two CCDD genomes. Comparative analysis of the karyotype of O.alta and O.latifolia chromosomes based on the Fluorescence in Situ Hybridization pictures showed vast differences. We propose that the genome of O.alta may be differentiated earlier in evolution and tend to be stable, while O.latifolia may still be in its evolutionary process. Based on the imbalance of evolution and differences of genomic structure, we propose that O.alta and O.latifolia can be divided into two wild rice species, which may be better conforming with their evolution characteristics. The mechanism and characteristics of 45s rDNA’s distribution in the chromosomes are discussed.},
year = {2018}
}
TY - JOUR T1 - FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes AU - Zhou Hui AU - Lan Weizhen AU - Qin Rui AU - Liu Hong AU - Li Gang Y1 - 2018/10/26 PY - 2018 N1 - https://doi.org/10.11648/j.ajaf.20180606.12 DO - 10.11648/j.ajaf.20180606.12 T2 - American Journal of Agriculture and Forestry JF - American Journal of Agriculture and Forestry JO - American Journal of Agriculture and Forestry SP - 169 EP - 175 PB - Science Publishing Group SN - 2330-8591 UR - https://doi.org/10.11648/j.ajaf.20180606.12 AB - Using Fluorescence in Situ Hybridization with 45S rDNA probes, we analyzed two wild rice species, Oryza alta and Oryza latifolia, both having CCDD genomes. The results showed that the hybridization signals of 45S rDNA in O.latifolia chromosome preparations were distributed on different chromosomes with the number of 10~16. The same signals in O.alta chromosome preparations were 6 and distributed on three pairs of homologous chromosomes: with two pairs in short chromosome arms and the other one pair in long chromosome arms. The number and positions of 45S rDNA signals are stable in O.alta genome, but there are some dynamic changes of signals in O.latifolia genome. Our observations indicated that there are distinct differences between the two CCDD genomes. Comparative analysis of the karyotype of O.alta and O.latifolia chromosomes based on the Fluorescence in Situ Hybridization pictures showed vast differences. We propose that the genome of O.alta may be differentiated earlier in evolution and tend to be stable, while O.latifolia may still be in its evolutionary process. Based on the imbalance of evolution and differences of genomic structure, we propose that O.alta and O.latifolia can be divided into two wild rice species, which may be better conforming with their evolution characteristics. The mechanism and characteristics of 45s rDNA’s distribution in the chromosomes are discussed. VL - 6 IS - 6 ER -
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