Camellia oleifera, a unique edible oil tree species in China, is of important economic value. However, the shortage of phosphorus in the soil is one of the important factors limiting the growth of C. oleifera. Here, we investigated the population size and composition of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of wild C. oleifera in Mountain Lushan, China. PSB were isolated using a dilution coating plate method and identified by 16S rRNA sequencing. The phosphate-solubilizing capability of the isolated PSB was evaluated by a semi-quantitative method (the ratio of phosphate solubilization halo diameter versus colony diameter). The results showed that large amounts of PSB existed in the rhizosphere soil of wild C. oleifera (0.28–1.08×107 CFU/g soil) and the population size of PSB differed from investigated trees. A total of 100 strains of PSB were isolated from the rhizosphere soil, belonging to Bacillus, Burkholderia, Pantoea, Paraburkholderia, and Pseudomonas, respectively. Of these strains, Burkholderia showed the highest isolation frequency and phosphate-solubilizing capability, accounting for 61% of the isolates. The phosphate solubilization index of 100 strains varied from 1.02 to 3.04 after a 6-day incubation, and Bacillus strains were easy to lose their phosphate-solubilizing capability during the incubation. Our result suggested that Burkholderia was the dominant genus of PSB in the rhizosphere of C. oleifera and could be utilized for facilitating the uptake of P.
| Published in | American Journal of Agriculture and Forestry (Volume 9, Issue 3) |
| DOI | 10.11648/j.ajaf.20210903.17 |
| Page(s) | 141-146 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Camellia oleifera, Phosphate-solubilizing Bacteria, 16S rRNA, Phosphate Solubilization Index
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APA Style
Qibiao Sun, Yanfen Liu, Yan Tang, Peiyu Zhang, Yao Tong, et al. (2021). The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan. American Journal of Agriculture and Forestry, 9(3), 141-146. https://doi.org/10.11648/j.ajaf.20210903.17
ACS Style
Qibiao Sun; Yanfen Liu; Yan Tang; Peiyu Zhang; Yao Tong, et al. The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan. Am. J. Agric. For. 2021, 9(3), 141-146. doi: 10.11648/j.ajaf.20210903.17
AMA Style
Qibiao Sun, Yanfen Liu, Yan Tang, Peiyu Zhang, Yao Tong, et al. The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan. Am J Agric For. 2021;9(3):141-146. doi: 10.11648/j.ajaf.20210903.17
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Download@article{10.11648/j.ajaf.20210903.17,
author = {Qibiao Sun and Yanfen Liu and Yan Tang and Peiyu Zhang and Yao Tong and Gang He and Xiaohong Ji and Zhenying He and Jianping Ouyang and Hongfang Zhang and Ye Chen},
title = {The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan},
journal = {American Journal of Agriculture and Forestry},
volume = {9},
number = {3},
pages = {141-146},
doi = {10.11648/j.ajaf.20210903.17},
url = {https://doi.org/10.11648/j.ajaf.20210903.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20210903.17},
abstract = {Camellia oleifera, a unique edible oil tree species in China, is of important economic value. However, the shortage of phosphorus in the soil is one of the important factors limiting the growth of C. oleifera. Here, we investigated the population size and composition of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of wild C. oleifera in Mountain Lushan, China. PSB were isolated using a dilution coating plate method and identified by 16S rRNA sequencing. The phosphate-solubilizing capability of the isolated PSB was evaluated by a semi-quantitative method (the ratio of phosphate solubilization halo diameter versus colony diameter). The results showed that large amounts of PSB existed in the rhizosphere soil of wild C. oleifera (0.28–1.08×107 CFU/g soil) and the population size of PSB differed from investigated trees. A total of 100 strains of PSB were isolated from the rhizosphere soil, belonging to Bacillus, Burkholderia, Pantoea, Paraburkholderia, and Pseudomonas, respectively. Of these strains, Burkholderia showed the highest isolation frequency and phosphate-solubilizing capability, accounting for 61% of the isolates. The phosphate solubilization index of 100 strains varied from 1.02 to 3.04 after a 6-day incubation, and Bacillus strains were easy to lose their phosphate-solubilizing capability during the incubation. Our result suggested that Burkholderia was the dominant genus of PSB in the rhizosphere of C. oleifera and could be utilized for facilitating the uptake of P.},
year = {2021}
}
TY - JOUR T1 - The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan AU - Qibiao Sun AU - Yanfen Liu AU - Yan Tang AU - Peiyu Zhang AU - Yao Tong AU - Gang He AU - Xiaohong Ji AU - Zhenying He AU - Jianping Ouyang AU - Hongfang Zhang AU - Ye Chen Y1 - 2021/05/31 PY - 2021 N1 - https://doi.org/10.11648/j.ajaf.20210903.17 DO - 10.11648/j.ajaf.20210903.17 T2 - American Journal of Agriculture and Forestry JF - American Journal of Agriculture and Forestry JO - American Journal of Agriculture and Forestry SP - 141 EP - 146 PB - Science Publishing Group SN - 2330-8591 UR - https://doi.org/10.11648/j.ajaf.20210903.17 AB - Camellia oleifera, a unique edible oil tree species in China, is of important economic value. However, the shortage of phosphorus in the soil is one of the important factors limiting the growth of C. oleifera. Here, we investigated the population size and composition of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of wild C. oleifera in Mountain Lushan, China. PSB were isolated using a dilution coating plate method and identified by 16S rRNA sequencing. The phosphate-solubilizing capability of the isolated PSB was evaluated by a semi-quantitative method (the ratio of phosphate solubilization halo diameter versus colony diameter). The results showed that large amounts of PSB existed in the rhizosphere soil of wild C. oleifera (0.28–1.08×107 CFU/g soil) and the population size of PSB differed from investigated trees. A total of 100 strains of PSB were isolated from the rhizosphere soil, belonging to Bacillus, Burkholderia, Pantoea, Paraburkholderia, and Pseudomonas, respectively. Of these strains, Burkholderia showed the highest isolation frequency and phosphate-solubilizing capability, accounting for 61% of the isolates. The phosphate solubilization index of 100 strains varied from 1.02 to 3.04 after a 6-day incubation, and Bacillus strains were easy to lose their phosphate-solubilizing capability during the incubation. Our result suggested that Burkholderia was the dominant genus of PSB in the rhizosphere of C. oleifera and could be utilized for facilitating the uptake of P. VL - 9 IS - 3 ER -
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