Background: Chukrasia Tabularis A. Fuss is a valuable woody plant and difficult in reproduction in nature. Objectives: There are a needs to conserve and develop for re-forest. Method: Manipulation of tissue culture via bioreactor techniques was the effective new way to resolve re-forest. Stems and leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium MS + BA (2mg/l) + PVP (500 mg/l) supplemented with 2,4D (2mg/l). Somatic cell suspension was cultured for initiation and for proliferation. On medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension was cultured in bioreactor for initiation and proliferation on the medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). Embryogenic suspension was stimulated on the medium MS + 2,4D (1mg/l) + CW (10%) + sucrose (45g/l) supplemented with BA (0.5mg/l) + NAA (0.1mg/l). In vitro shoots of Chukrasia Tabularis a. Fuss were regeneration on the medium MS + 2,4D (1mg/l) + CW (10%) + sucrose (45g/l) supplemented with TDZ (0,5mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 26±2°C, 11,1-22,2μmol/m2/s, and 30rpm. Results: Micropropagation of Chukrasia Tabularis a. Fuss by bioreactor technique was set up to produce 4,680 plantlets per one liter of somatic embryogenesis suspension Conclusion: a process of rapid industrial multiplication of woody plants by bioreactor technology has been built.
| Published in | American Journal of Agriculture and Forestry (Volume 10, Issue 3) |
| DOI | 10.11648/j.ajaf.20221003.15 |
| Page(s) | 111-117 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Chukrasia Tabularis A. Fuss, Micropropagation, Bioreactor Technology
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APA Style
Tran Van Minh. (2022). Industrial Propagation of Chukrasia Tabularis A. Fuss by Bioreactor Technique. American Journal of Agriculture and Forestry, 10(3), 111-117. https://doi.org/10.11648/j.ajaf.20221003.15
ACS Style
Tran Van Minh. Industrial Propagation of Chukrasia Tabularis A. Fuss by Bioreactor Technique. Am. J. Agric. For. 2022, 10(3), 111-117. doi: 10.11648/j.ajaf.20221003.15
AMA Style
Tran Van Minh. Industrial Propagation of Chukrasia Tabularis A. Fuss by Bioreactor Technique. Am J Agric For. 2022;10(3):111-117. doi: 10.11648/j.ajaf.20221003.15
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Download@article{10.11648/j.ajaf.20221003.15,
author = {Tran Van Minh},
title = {Industrial Propagation of Chukrasia Tabularis A. Fuss by Bioreactor Technique},
journal = {American Journal of Agriculture and Forestry},
volume = {10},
number = {3},
pages = {111-117},
doi = {10.11648/j.ajaf.20221003.15},
url = {https://doi.org/10.11648/j.ajaf.20221003.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20221003.15},
abstract = {Background: Chukrasia Tabularis A. Fuss is a valuable woody plant and difficult in reproduction in nature. Objectives: There are a needs to conserve and develop for re-forest. Method: Manipulation of tissue culture via bioreactor techniques was the effective new way to resolve re-forest. Stems and leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium MS + BA (2mg/l) + PVP (500 mg/l) supplemented with 2,4D (2mg/l). Somatic cell suspension was cultured for initiation and for proliferation. On medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension was cultured in bioreactor for initiation and proliferation on the medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). Embryogenic suspension was stimulated on the medium MS + 2,4D (1mg/l) + CW (10%) + sucrose (45g/l) supplemented with BA (0.5mg/l) + NAA (0.1mg/l). In vitro shoots of Chukrasia Tabularis a. Fuss were regeneration on the medium MS + 2,4D (1mg/l) + CW (10%) + sucrose (45g/l) supplemented with TDZ (0,5mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 26±2°C, 11,1-22,2μmol/m2/s, and 30rpm. Results: Micropropagation of Chukrasia Tabularis a. Fuss by bioreactor technique was set up to produce 4,680 plantlets per one liter of somatic embryogenesis suspension Conclusion: a process of rapid industrial multiplication of woody plants by bioreactor technology has been built.},
year = {2022}
}
TY - JOUR T1 - Industrial Propagation of Chukrasia Tabularis A. Fuss by Bioreactor Technique AU - Tran Van Minh Y1 - 2022/06/20 PY - 2022 N1 - https://doi.org/10.11648/j.ajaf.20221003.15 DO - 10.11648/j.ajaf.20221003.15 T2 - American Journal of Agriculture and Forestry JF - American Journal of Agriculture and Forestry JO - American Journal of Agriculture and Forestry SP - 111 EP - 117 PB - Science Publishing Group SN - 2330-8591 UR - https://doi.org/10.11648/j.ajaf.20221003.15 AB - Background: Chukrasia Tabularis A. Fuss is a valuable woody plant and difficult in reproduction in nature. Objectives: There are a needs to conserve and develop for re-forest. Method: Manipulation of tissue culture via bioreactor techniques was the effective new way to resolve re-forest. Stems and leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium MS + BA (2mg/l) + PVP (500 mg/l) supplemented with 2,4D (2mg/l). Somatic cell suspension was cultured for initiation and for proliferation. On medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension was cultured in bioreactor for initiation and proliferation on the medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). Embryogenic suspension was stimulated on the medium MS + 2,4D (1mg/l) + CW (10%) + sucrose (45g/l) supplemented with BA (0.5mg/l) + NAA (0.1mg/l). In vitro shoots of Chukrasia Tabularis a. Fuss were regeneration on the medium MS + 2,4D (1mg/l) + CW (10%) + sucrose (45g/l) supplemented with TDZ (0,5mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 26±2°C, 11,1-22,2μmol/m2/s, and 30rpm. Results: Micropropagation of Chukrasia Tabularis a. Fuss by bioreactor technique was set up to produce 4,680 plantlets per one liter of somatic embryogenesis suspension Conclusion: a process of rapid industrial multiplication of woody plants by bioreactor technology has been built. VL - 10 IS - 3 ER -
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