The Valuation of Genetic Diversity in <i>Tarchonanthus camphoratus</i> Plant Using ISSR Markers in Taif, KSA

Hatim Matouq Alyasi; Rahmah Nasser Alqthanin

doi:doi:10.11648/j.ajaf.20190706.15

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The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA

Hatim Matouq Alyasi, Rahmah Nasser Alqthanin

Published in American Journal of Agriculture and Forestry (Volume 7, Issue 6)

Received: 1 September 2019 Accepted: 5 October 2019 Published: 8 November 2019

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Abstract

Genetic variation among T. camphoratus individual plant samples collected from Taif region, Saudi Arabia was assessed using inter-simple sequence repeat (ISSR) markers. Eleven ISSR primers were used to evaluate T. camphoratus samples. The number of polymorphic bands per primer varied from 5 to 16, with 10.3 bands per primer on average. Nei’s genetic distance between T. camphoratus samples ranged from 0.03 to 0.94. According to genetic similarity and intraspecific differentiation, the 15 T. camphoratus were grouped into two main different clusters with about 0.67 genetic similarity. It was not possible to classify the samples according to their geographic origin, showing that there is no structure in the gene bank. Cluster analysis is indicating good resolution of genetic diversity in Tarchonanthus germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection, biodiversity and to assist breeding and conservation strategies.

Published in	American Journal of Agriculture and Forestry (Volume 7, Issue 6)
DOI	10.11648/j.ajaf.20190706.15
Page(s)	277-281
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Tarchonanthus camphoratus, Genetic Variability, Molecular Markers, Genetic Resources

References

[1]	Acocks JPH, 1988. Veld types of South Africa. Edn. 3. Memoirs of the Botanical Survey of South Africa: 57.
[2]	Pope GV, 1992. Compositae. Flora Zambesiaca 6: 9-11.
[3]	Herman PPJ, 2002. Revision of the Tarchonanathus camphoratus complex (Asteraceae- Tarchonantheae) in Southern Africa. Bothalia 32: 21-28.
[4]	Huyssteen M, Milne PJ, Campbell EE, van de Venter M, 2011. Antidiabetic and cytotoxicity screening of five medicinal plants used by traditional African health practitioners in the Nelson Mandela Metropole, South Africa. African Journal of Traditional, Complementary and Alternative Medicines, 8: 150-156.
[5]	Njogu MK, Josphat CM, Marcel K, Reto B, Thomas JS, 2018. Antiprotozoal Sesquiterpene Lactones and Other Constituents from Tarchonanthus camphoratus and Schkuhria pinnata. Journal of Natural Products 81 (1): 124-130.
[6]	Van-Vuuren SF, Viljoen AM, 2009. Interaction between the non-volatile and volatile fractions on the antimicrobial activity of Tarchonanthus camphoratus. South African Journal of Botany, 75: 505–509.
[7]	Millan T1, Osuna F, Cobos S, Torres AM, Cubero JI, 1996. Using RAPDs to study phylogenetic relationships in Rosa. Theor Appl Genet., 92 (2): 273-7. doi: 10.1007/BF00223385.
[8]	Atienza SG, Torres AM, Millan T, Cubero J I, 2005. Genetic diversity in Rosa as revealed by RAPDs, Agric Conspec Sci, 70: 75-85.
[9]	Crespel L, Pernet A, Le Bris M, Gudin S, Hibrand Saint Oyant L, 2009. Application of ISSRs for cultivar identification and assessment of genetic relationships in rose. Plant Breeding 12: 501-506.
[10]	Zhang LH, Byrne DH, Ballard RE, Rajapakse S, 2006. Microsatellite marker development in rose and its application in tetraploid mapping. J Am Soc Hortic Sci 131: 380-387.
[11]	Golkar P, Arzani A, Rezaei AM, 2011. Genetic variation in safflower (Carthamus tinctorious L.) for seed quality-related traits and intersimple sequence repeat (ISSR) markers. Int J Mol Sci 12: 2664-2677.
[12]	Nadeem M, Wang X, Akond M, Awan FS, Riaz A, Younis A, 2014. Hybrid identification, morphological evaluation and genetic diversity analysis of Rosa x hybrida by SSR markers. Australian Journal of Crop Science 8: 183-190.
[13]	Hassan MM, Gaber A, El-Hallous E, 2014. Molecular and Morphological Characterization of Trichoderma harzianum from different Egyptian Soils. Wulfenia Journal 21: 80-96.
[14]	Luo C, He XH, Chen H, Ou SJ, Gao MP, 2010. Analysis of diversity and relationships among mango cultivars using Start Codon Targeted (SCoT) markers. Biochem. Syst. Ecol. 38: 1176–1184.
[15]	Mulpuri S, Muddanuru T, Francis G, 2013. Start codon targeted (SCoT) polymorphism in toxic and non-toxic accessions of Jatropha curcas L. and development of a codominant SCAR marker. Plant Sci., 207: 117–127.
[16]	Xiong F, Zhong R, Han Z, Jiang J, He L, Zhuang W, Tang R, 2011. Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes. Mol. Biol. Rep., 38: 3487–3494.
[17]	Rohlf FJ, 2000. NTSYS-PC numerical taxonomy and multivariate analysis system, Version 2.1. Exeter Software, Setauket, New York.
[18]	Nasser A, Alia A, Mohamed A, Al-Fatimib R, Crouchc A, Denkertd, A, William N. S, Wessjohannd L., 2013. Antimicrobial, Antioxidant, and Cytotoxic Activities of the Essential Oil of Tarchonanthus camphoratus. Natural product communications. (5): 863-686.
[19]	Schanzer IA, Vagina AV, 2007. ISSR (Inter Simple Sequence Repeat) Markers Reveal Natural Intersectional Hybridization in Wild Roses [Rosa (L.), Sect. Caninae (DC.) Ser. and Sect. Cinnamomeae (DC.) Ser.], Wulfenia, 14: 1-14.
[20]	El-Assal S, El-Awady, M, El-Tarras A, Shehab G, 2014. Assessing the genetic relationship of Taif rose with some rose genotypes (Rosa sp.) Based on random amplified polymorphic DNA, inter simple sequence repeat and simple sequence repeat markers American Journal of Biochemistry and Biotechnology 10 (1): 88-98.
[21]	Liu B, Wendel JF, 2001. Inter simple sequence repeat (ISSR) polymorphisms as a genetic marker system in cotton. Mol Ecol Notes 1: 205-208.
[22]	Ullah S, Hasan Z, Aziz F, Amir I, Muhammad I, 2015. Diversity of Edible Fishes at Rhound Stream District Dir Lower, Khyber Pakhtunkhwa Pakistan. International Journal of Innovation and Applied Studies 10 (2): 466-472.

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APA Style

Hatim Matouq Alyasi, Rahmah Nasser Alqthanin. (2019). The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA. American Journal of Agriculture and Forestry, 7(6), 277-281. https://doi.org/10.11648/j.ajaf.20190706.15

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ACS Style

Hatim Matouq Alyasi; Rahmah Nasser Alqthanin. The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA. Am. J. Agric. For. 2019, 7(6), 277-281. doi: 10.11648/j.ajaf.20190706.15

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AMA Style

Hatim Matouq Alyasi, Rahmah Nasser Alqthanin. The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA. Am J Agric For. 2019;7(6):277-281. doi: 10.11648/j.ajaf.20190706.15

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@article{10.11648/j.ajaf.20190706.15,
  author = {Hatim Matouq Alyasi and Rahmah Nasser Alqthanin},
  title = {The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA},
  journal = {American Journal of Agriculture and Forestry},
  volume = {7},
  number = {6},
  pages = {277-281},
  doi = {10.11648/j.ajaf.20190706.15},
  url = {https://doi.org/10.11648/j.ajaf.20190706.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20190706.15},
  abstract = {Genetic variation among T. camphoratus individual plant samples collected from Taif region, Saudi Arabia was assessed using inter-simple sequence repeat (ISSR) markers. Eleven ISSR primers were used to evaluate T. camphoratus samples. The number of polymorphic bands per primer varied from 5 to 16, with 10.3 bands per primer on average. Nei’s genetic distance between T. camphoratus samples ranged from 0.03 to 0.94. According to genetic similarity and intraspecific differentiation, the 15 T. camphoratus were grouped into two main different clusters with about 0.67 genetic similarity. It was not possible to classify the samples according to their geographic origin, showing that there is no structure in the gene bank. Cluster analysis is indicating good resolution of genetic diversity in Tarchonanthus germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection, biodiversity and to assist breeding and conservation strategies.},
 year = {2019}
}

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TY - JOUR
T1 - The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA
AU - Hatim Matouq Alyasi
AU - Rahmah Nasser Alqthanin
Y1 - 2019/11/08
PY - 2019
N1 - https://doi.org/10.11648/j.ajaf.20190706.15
DO - 10.11648/j.ajaf.20190706.15
T2 - American Journal of Agriculture and Forestry
JF - American Journal of Agriculture and Forestry
JO - American Journal of Agriculture and Forestry
SP - 277
EP - 281
PB - Science Publishing Group
SN - 2330-8591
UR - https://doi.org/10.11648/j.ajaf.20190706.15
AB - Genetic variation among T. camphoratus individual plant samples collected from Taif region, Saudi Arabia was assessed using inter-simple sequence repeat (ISSR) markers. Eleven ISSR primers were used to evaluate T. camphoratus samples. The number of polymorphic bands per primer varied from 5 to 16, with 10.3 bands per primer on average. Nei’s genetic distance between T. camphoratus samples ranged from 0.03 to 0.94. According to genetic similarity and intraspecific differentiation, the 15 T. camphoratus were grouped into two main different clusters with about 0.67 genetic similarity. It was not possible to classify the samples according to their geographic origin, showing that there is no structure in the gene bank. Cluster analysis is indicating good resolution of genetic diversity in Tarchonanthus germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection, biodiversity and to assist breeding and conservation strategies.
VL - 7
IS - 6
ER -

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Author Information

Hatim Matouq Alyasi

Biological Science, Taif Unoversity, Taif, Saudi Arabia
Rahmah Nasser Alqthanin

Biological Science, King Khalid University, Abha, Saudi Arabia

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APA Style

Hatim Matouq Alyasi, Rahmah Nasser Alqthanin. (2019). The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA. American Journal of Agriculture and Forestry, 7(6), 277-281. https://doi.org/10.11648/j.ajaf.20190706.15

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ACS Style

Hatim Matouq Alyasi; Rahmah Nasser Alqthanin. The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA. Am. J. Agric. For. 2019, 7(6), 277-281. doi: 10.11648/j.ajaf.20190706.15

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AMA Style

Hatim Matouq Alyasi, Rahmah Nasser Alqthanin. The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA. Am J Agric For. 2019;7(6):277-281. doi: 10.11648/j.ajaf.20190706.15

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@article{10.11648/j.ajaf.20190706.15,
  author = {Hatim Matouq Alyasi and Rahmah Nasser Alqthanin},
  title = {The Valuation of Genetic Diversity in Tarchonanthus camphoratus Plant Using ISSR Markers in Taif, KSA},
  journal = {American Journal of Agriculture and Forestry},
  volume = {7},
  number = {6},
  pages = {277-281},
  doi = {10.11648/j.ajaf.20190706.15},
  url = {https://doi.org/10.11648/j.ajaf.20190706.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20190706.15},
  abstract = {Genetic variation among T. camphoratus individual plant samples collected from Taif region, Saudi Arabia was assessed using inter-simple sequence repeat (ISSR) markers. Eleven ISSR primers were used to evaluate T. camphoratus samples. The number of polymorphic bands per primer varied from 5 to 16, with 10.3 bands per primer on average. Nei’s genetic distance between T. camphoratus samples ranged from 0.03 to 0.94. According to genetic similarity and intraspecific differentiation, the 15 T. camphoratus were grouped into two main different clusters with about 0.67 genetic similarity. It was not possible to classify the samples according to their geographic origin, showing that there is no structure in the gene bank. Cluster analysis is indicating good resolution of genetic diversity in Tarchonanthus germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection, biodiversity and to assist breeding and conservation strategies.},
 year = {2019}
}

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