Vietnam has planning in re-forest of Chukrasia tabularis, a special-use forest and re-culture of Coffea sp., an industrial crops. The first barrier is seedlings for supplying higher demand from farmers. Using thin cell layer culture method is one of the ways to resolve seedlings of woody species. Results gave capacities using thin cell layer of shoot-tips in regeneration and using them for far propagation. Chukrasia tabularis A. Juss: Shoot tip from the pot was used as material to establish in vitro plant and was cultured on medium MS + BA (0.1mg/l) to rise up 5.7 shoots/sample and shoot height 12mm after 30 days cultivation. In vitro plantlets were used as materials for thin cell layer cultivation. Samples of leaves and slices of shoot tip were cultured on medium MS + BA (0.1mg/l) + CW (10%) to initiate 2.2 shoots/leaf sample and 5.8 shoots/slices of shoot tip sample. Using 2 genotypes from Con Dao and Lang Son to show not different in shoot initiation of 5.4-5.6 shoots/sample. Shoots were regenerated and rooted well on medium MS + NAA (0.1mg/l). The thin cell layer culture of Chukrasia tabularis A. Juss was established. Coffea sp.: Shoot tip from the pot plant was used as material for set up in vitro plantlet by cultiavting on medium WPM + BA (0.1mg/l) after 45 days. In vitro plantlets were used as material for thin cell layer on medium MS + Adenin (20mg/l) + kinetin (1mg/l) + BA (3mg/l) and to rise up 5.2 shoots/leaf sample (1cm2) and 6.2 shoots/shoot tip meristem sample after 45 days cultivation. Using 2 clones of Coffea sp. as C. robusta and C. arabica in cultivation to imitiate 4-6 shoots/robusta leaf sample and 3-5 shoots/arabica leaf sample. Plantlets were regenerated and rooted well on medium WPM + IBA (0.6mg/l). The thin cell layer culture of Coffea sp. was established.
| Published in | American Journal of Agriculture and Forestry (Volume 11, Issue 4) |
| DOI | 10.11648/j.ajaf.20231104.14 |
| Page(s) | 143-150 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Chukrasia tabularis A. Juss, Coffea sp., Thin Cell Layer (TCL), C. robusta, C. arabica
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APA Style
Tran Van Minh. (2023). Thin Cell Layer Culture of Chukrasia tabularis A. Fuss and Coffea sp.. American Journal of Agriculture and Forestry, 11(4), 143-150. https://doi.org/10.11648/j.ajaf.20231104.14
ACS Style
Tran Van Minh. Thin Cell Layer Culture of Chukrasia tabularis A. Fuss and Coffea sp.. Am. J. Agric. For. 2023, 11(4), 143-150. doi: 10.11648/j.ajaf.20231104.14
AMA Style
Tran Van Minh. Thin Cell Layer Culture of Chukrasia tabularis A. Fuss and Coffea sp.. Am J Agric For. 2023;11(4):143-150. doi: 10.11648/j.ajaf.20231104.14
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Download@article{10.11648/j.ajaf.20231104.14,
author = {Tran Van Minh},
title = {Thin Cell Layer Culture of Chukrasia tabularis A. Fuss and Coffea sp.},
journal = {American Journal of Agriculture and Forestry},
volume = {11},
number = {4},
pages = {143-150},
doi = {10.11648/j.ajaf.20231104.14},
url = {https://doi.org/10.11648/j.ajaf.20231104.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20231104.14},
abstract = {Vietnam has planning in re-forest of Chukrasia tabularis, a special-use forest and re-culture of Coffea sp., an industrial crops. The first barrier is seedlings for supplying higher demand from farmers. Using thin cell layer culture method is one of the ways to resolve seedlings of woody species. Results gave capacities using thin cell layer of shoot-tips in regeneration and using them for far propagation. Chukrasia tabularis A. Juss: Shoot tip from the pot was used as material to establish in vitro plant and was cultured on medium MS + BA (0.1mg/l) to rise up 5.7 shoots/sample and shoot height 12mm after 30 days cultivation. In vitro plantlets were used as materials for thin cell layer cultivation. Samples of leaves and slices of shoot tip were cultured on medium MS + BA (0.1mg/l) + CW (10%) to initiate 2.2 shoots/leaf sample and 5.8 shoots/slices of shoot tip sample. Using 2 genotypes from Con Dao and Lang Son to show not different in shoot initiation of 5.4-5.6 shoots/sample. Shoots were regenerated and rooted well on medium MS + NAA (0.1mg/l). The thin cell layer culture of Chukrasia tabularis A. Juss was established. Coffea sp.: Shoot tip from the pot plant was used as material for set up in vitro plantlet by cultiavting on medium WPM + BA (0.1mg/l) after 45 days. In vitro plantlets were used as material for thin cell layer on medium MS + Adenin (20mg/l) + kinetin (1mg/l) + BA (3mg/l) and to rise up 5.2 shoots/leaf sample (1cm2) and 6.2 shoots/shoot tip meristem sample after 45 days cultivation. Using 2 clones of Coffea sp. as C. robusta and C. arabica in cultivation to imitiate 4-6 shoots/robusta leaf sample and 3-5 shoots/arabica leaf sample. Plantlets were regenerated and rooted well on medium WPM + IBA (0.6mg/l). The thin cell layer culture of Coffea sp. was established.},
year = {2023}
}
TY - JOUR T1 - Thin Cell Layer Culture of Chukrasia tabularis A. Fuss and Coffea sp. AU - Tran Van Minh Y1 - 2023/07/17 PY - 2023 N1 - https://doi.org/10.11648/j.ajaf.20231104.14 DO - 10.11648/j.ajaf.20231104.14 T2 - American Journal of Agriculture and Forestry JF - American Journal of Agriculture and Forestry JO - American Journal of Agriculture and Forestry SP - 143 EP - 150 PB - Science Publishing Group SN - 2330-8591 UR - https://doi.org/10.11648/j.ajaf.20231104.14 AB - Vietnam has planning in re-forest of Chukrasia tabularis, a special-use forest and re-culture of Coffea sp., an industrial crops. The first barrier is seedlings for supplying higher demand from farmers. Using thin cell layer culture method is one of the ways to resolve seedlings of woody species. Results gave capacities using thin cell layer of shoot-tips in regeneration and using them for far propagation. Chukrasia tabularis A. Juss: Shoot tip from the pot was used as material to establish in vitro plant and was cultured on medium MS + BA (0.1mg/l) to rise up 5.7 shoots/sample and shoot height 12mm after 30 days cultivation. In vitro plantlets were used as materials for thin cell layer cultivation. Samples of leaves and slices of shoot tip were cultured on medium MS + BA (0.1mg/l) + CW (10%) to initiate 2.2 shoots/leaf sample and 5.8 shoots/slices of shoot tip sample. Using 2 genotypes from Con Dao and Lang Son to show not different in shoot initiation of 5.4-5.6 shoots/sample. Shoots were regenerated and rooted well on medium MS + NAA (0.1mg/l). The thin cell layer culture of Chukrasia tabularis A. Juss was established. Coffea sp.: Shoot tip from the pot plant was used as material for set up in vitro plantlet by cultiavting on medium WPM + BA (0.1mg/l) after 45 days. In vitro plantlets were used as material for thin cell layer on medium MS + Adenin (20mg/l) + kinetin (1mg/l) + BA (3mg/l) and to rise up 5.2 shoots/leaf sample (1cm2) and 6.2 shoots/shoot tip meristem sample after 45 days cultivation. Using 2 clones of Coffea sp. as C. robusta and C. arabica in cultivation to imitiate 4-6 shoots/robusta leaf sample and 3-5 shoots/arabica leaf sample. Plantlets were regenerated and rooted well on medium WPM + IBA (0.6mg/l). The thin cell layer culture of Coffea sp. was established. VL - 11 IS - 4 ER -
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